Stroke Model (MCAO)
The middle cerebral artery occlusion (MCAO) model is a commonly used technique for the creation of a stroke model. Laser Doppler is typically used in order to confirm that occlusion has taken place and ischaemia has occurred. With successful surgery novel therapies can be tested to assess the impact on stroke recovery. Typical published figures demonstrate that a reduction in monitored blood flow of up to 90% can be observed following successful occlusion.
We recommend the moorVMS-LDF, moorVMS-PC software, VP10M200ST master probe with P10d single fibre slave probe with corresponding probe holders. Surgical glue and accelerator can be used to securely fix the probes in place. For thicker skulls (older/ larger rodents) the single fibre solution may not penetrate the skull sufficiently and in this case the twin fibre VP14-CBF probe is used. For those using a stereotaxic frame we recommend the use of needle probes, the VP3, VP4 or VP5.
Contact us to discuss your specific needs and to request your copy of our free Application Note which includes a detailed experimental method and practical suggestions. We also offer no obligation evaluation loans so you can test the equipment in your facility.
Gibson C. L., Constantin D., Prior M. J. W., Bath P. M. W., Murphy S. P. 2005 Progesterone suppresses the inflammatory response and nitric oxide synthase-2 expression following cerebral ischemia. Experimental Neurology, 193, pp 522– 530.
Fowler J. C., Gervitz L. M., Hamilton M. E., Walker J. A. 2003 Systemic hypoxia and the depression of synaptic transmission in rat hippocampus after carotid artery occlusion. Journal of Physiology, 550 (Pt 3), pp 961-72
Dinapoli V. A., Rosen C. L., Nagamine T., Crocco T. J. 2006 Selective MCA occlusion: A precise embolic stroke model. Neurosci Methods, 154 (1-2), pp 233-8
VP10M200ST 'master' probemoorVMS-PC
Advanced aquisition and analyses functions to collect signals and automate control protocol modulesP10d
Single plastic fibre, sleeving 0.5mm diameter, with a detachable optic connectorVP14-CBF
Small shank stainless steel needle probe for pre-clinical CBF measurements