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Applications

Cortical Spreading Depression (CSD)

Cortical spreading depression (CSD), also known as cortical spreading depolarization, describes a wave of neuronal depolarization associated with influx of cat-ions and water which blocks normal cerebral activity. CSD induces changes to microvascular tone with the vasoconstriction phase (Cortical Spreading Ischeamia, CSI) with associated risk of progressive damage. The waves move relatively slowly and experimental areas of the cerebral cortex are relatively small.

Anaesthesia choice has been found to have a significant impact on cerebral auto-regulation; investigations found that auto-regulation of cerebral tissue blood flow, assessed by moorFLPI-2, was preserved with Ethomidate down to about 27mmHg mean arterial pressure (MAP) whereas with several other anaesthetics, cerebral auto-regulation was abolished and a linear relationship between cerebral tissue blood flow and MAP was observed.

Equipment recommendations

The scan duration of a high resolution (100µm/pixel) LDI (moorLDI2-HIR) is acceptable. For higher resolution (10µm/pixel) and greater speed, speckle imaging with moorFLPI-2, operating in temporal mode, is the better choice. To aid imaging stability and manipulation, the moorFLPI-2 should be mounted on a moorFLPI-microstand. Laser Doppler monitoring can be used to observe the CSD wave transit a single point. These measurements can be made with the moorVMS-LDF and needle probes (e.g. VP3) held in position by a micromanipulator or by using a wet-stick probe holder at the probe tip (PHN-WS).

What's next?

Contact us to discuss your specific needs and to request your copy of our free Application Note which includes a detailed experimental method and practical suggestions. We also offer no obligation on-site visits so you can test the equipment in your facility.

References

Wang Z, Schuler B, Vogel O, Arras M, Vogel J. What is the optimal anesthetic protocol for measurements of cerebral autoregulation in spontaneously breathing mice? Exp Brain Res. 2010; 207(3-4): 249-258.

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